Microsatellite Enrichment in Organisms with Large Genomes (Allium cepa L.)

Microsatellite enrichment in organisms with large genomes (Allium cepa L.).

To exploit the polymorphism of repeat numbers in short tandem repeat (STR) sequences (microsatellites) as molecular markers, STRs must be isolated and PCR primers must be developed in flanking sequences. In species with large genomes such as Allium cepa L. (onion and shallot), an efficient selection procedure for genomic fragments containing STRs is a crucial step. Here we describe a nonradioac...

Assessment report on Allium cepa L., bulbus

Effect of sulphur application in onion (Allium cepa L.)

An experiment was conducted to study the effect of sulphur application on growth, yield and quality of onion at Horticulture Farm of Institute of Agriculture, Visva-Bharati, Sriniketan. The treatments consisted of six incremental doses of sulphur application (10, 20, 30, 40, 50 and 60 kg ha-1) and no sulphur application (control, 0 kg S ha-1). Treatments were arranged in Randomized Block Design...

Estimation of Genetic Diversity in Onion (Allium cepa L.)

The experiment was conducted at the field laboratory of the Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during November 2008 to March 2009 to estimate the genetic diversity of exotic and local onion. Ten genotypes of onion were evaluated for 16 characters in a randomized complete block design to study the genetic divergence throu...

Biomonitoring hospital effluents by the Allium cepa L. test.

Hospital effluents are serious problems in developing countries like Brazil, and when not treated adequately, can cause mutagenic effects on live organisms. Biomonitors, like Allium cepa L., which is one of the most used plant species when monitoring effluent genotoxicity, have been used to alert the world population about environmental contamination and genotoxic chemical emissions. The Allium...